100 Days of Immunology

Day	Title
1	Why I teach here
2	Immune System – The Only Cellular System
3	Innate vs. Adaptive Immunity: The Fast and The Learned
4	Hematopoiesis – where Immune Cells are Coming From
5	Cytokines – The Language of Immune Signaling
6	Antigen Presentation Basics
7	Immunological Memory and The Immune Synapse
8	Barriers of Immunity
9	Inflammation 101
10	How We Study Immunology in the Labs
11	Neutrophils: Rapid Respondents
12	Monocytes and Macrophages: Differentiation and Phagocytosis
13	Dendritic cells: Antigen Presentation
14	Natural Killer Cells: The Innate Executioners
15	B Cells At Work: The Makers of Antibodies
16	T Helper (CD4+) Cells: Conductors of The Immune Orchestra
17	Cytotoxic (CD8+) Cells: Precision Assasins of The Immune System
18	Tregs: Masters of Suppression and Tolerance
19	NKT Cells: Bridging Innate and Adaptive Immunity
20	ILCs: Emerging Roles in Immunity
21	Eosinophils: Allergies and Parasites
22	Basophils and Mast Cells: Allergy Mediators
23	γδ T cells: Non-Classical T Cell Defenders
24	Overview: Recap of all Cell Types
25	Reflections on 24 Days of Learning
26	Mapping Immune Cells with Flow Cytometry
27	Fluorophore Fundamentals, panel Design and Spectral Flow Visualization
28	Flow Cytometry Controls: FMO, Isotype, and Experimental Validation
29	Visualizing Data: Dot Plots, Histograms and Gating Approaches
30	Troubleshooting Flow Cytometry
31	Cell Culture Essentials: Sterility and Handling
32	Preventing Contamination In Cell Culture
33	Understanding Adherent and Suspension Cell Culture
34	THP1 Differentiation into M1 and M2 Macrophages
35	Understanding ELISA: Mechanism and Uses
36	Mucus Secretion Assay: Beyond Classical ELISA
37	Western Blot Basics: From Gel To Band Interpretation
38	qPCR Basics: From RNA to Quantification
39	ELISpot Basics: Detecting Cytokine-secreting Cells with Precision
40	Fundamentals of Fluorescence Microscopy
41	Live Imaging: Watching Cells in Motion
42	Unlocking The Power of Multiplexing: From Ctokine profiling to CAR-T Potency
43	Cell Culture Crisis: Wh Are My Cells Dying
44	Lab Safety and Good Scientifc Practice
45	Lab Methods that Test Your Patience
46	Mouse Breeding Basics: Building Colonies, Tracing Generations
47	Drug Dosing for Mice: Calculations and Practical Considerations
48	Mouse Injections: IV, IP, SC and Beyond
49	Sampling and Visualization: Blood and Organ Concepts in Mice
50	Mastering Mouse Handling
51	Introduction to Cancer Immunology
52	Immune Checkpoints: Balancing Tolerance and Tumor Immunity
53	Adoptive Cell Therapy: Empowering The Immune Army
54	CAR-T Overview
55	FDA Approved CAR-Ts
56	CAR-T Side Effects
57	CAR-T in Solid Tumors: Overcoming The TME
58	Next Gen CAR-Ts: Synthetic Biology and Precision cell Therapies
59	Oncolytic Viruses: Turning Infection into Immunity
60	BiTEs: Bridging T Cells and Tumors for Precision Immunotherapy
61	Natural Killer Cell-based Therapies
62	Reprogramming The Defenders: Macrophage-based Cell Therapies
63	The Tumor Microenvironment: Layers of Immunosuppression and Resistance
64	Neoantigen Vaccines: Personalized Immunotherapy
65	Antigen Escape and Tumor Heterogeneity
66	Combination Regimens in Cancer Immunotherapy
67	From Bench To Bedside: Clinical Trial Stages in Modern Cancer
68	Investigational Medicinal Products in the EU
69	Individueller Heilversuch in Germany: When Innovation meets unmet Clinical Need
70	Recap of Immuno-oncology Series
71	Principles of Molecular Cloning
72	CRISPR in Immunology
73	Synthetic Biology beyond CAR-Ts: Mechanistic Principles
74	CAR Design Engineering
75	Optogenetics in Cellular and Immunotherapy
76	Organoids in Immunology: Modeling Human Immunity in the Dish
77	Microfluidics: Microenvironment Modeling and Precision Immunology
78	CyTOF: Mass Spectrometry for High-Dimensional Immune Profiling
79	Spatial Transcriptomics: Decoding Tissue-level Gene Expression Landscapes
80	Principles and Applications of Single-Cell RNA Sequencing in Immunology
81	Decoding Epigenetic Landscapes with ATAC-seq: from Regulatory Elements to Immune Function
82	Translating Methods to Immunology Research Questions
83	Hot vs. Cold Tumors: Immune-inflamed and Immune-excluded Cancers
84	Viruses as Vectors: Harnessing Viral Platforms for Gene Delivery in Immunology
85	Shuffling TCRs and Antibodies
86	Most Notable Immunotherapy Clinical Trials
87	Lab Automation: Streamlining Experiments for Modern immunology
88	3D Printing in Biomedical Research
89	Essential Laboratory Acronyms in Biomedical Research
90	Immune-Cardiovascular Crosstalk in Health and Disease
91	Allergies: From Antigen Exposure to Immune Outcome
92	Mechanisms Matter: A Recap of Molecular and Cellular Immunology
93	Feeder Cells in Cell Culture: Supporting Cellular Growth, Maintenance, and Differentiation
94	Feeder-independent Cell Expansion: Advancing Scalable and Controlled Cell Culture Systems
95	T Cell Exhaustion vs. Senescence
96	The Reductionist Approach in Immunology: Dissecting Complex Systems into Mechanistic Units
97	Non-Hodgkin Lymphoma: Immunological Origins, Molecular Heterogeneity, and Therapeutic Implications
98	Immunology Abbreviations
99	Reflections after 99 Days of Teaching Immunology
100	What´s Next