| Day | Title |
| 1 | Why I teach here |
| 2 | Immune System – The Only Cellular System |
| 3 | Innate vs. Adaptive Immunity: The Fast and The Learned |
| 4 | Hematopoiesis – where Immune Cells are Coming From |
| 5 | Cytokines – The Language of Immune Signaling |
| 6 | Antigen Presentation Basics |
| 7 | Immunological Memory and The Immune Synapse |
| 8 | Barriers of Immunity |
| 9 | Inflammation 101 |
| 10 | How We Study Immunology in the Labs |
| 11 | Neutrophils: Rapid Respondents |
| 12 | Monocytes and Macrophages: Differentiation and Phagocytosis |
| 13 | Dendritic cells: Antigen Presentation |
| 14 | Natural Killer Cells: The Innate Executioners |
| 15 | B Cells At Work: The Makers of Antibodies |
| 16 | T Helper (CD4+) Cells: Conductors of The Immune Orchestra |
| 17 | Cytotoxic (CD8+) Cells: Precision Assasins of The Immune System |
| 18 | Tregs: Masters of Suppression and Tolerance |
| 19 | NKT Cells: Bridging Innate and Adaptive Immunity |
| 20 | ILCs: Emerging Roles in Immunity |
| 21 | Eosinophils: Allergies and Parasites |
| 22 | Basophils and Mast Cells: Allergy Mediators |
| 23 | γδ T cells: Non-Classical T Cell Defenders |
| 24 | Overview: Recap of all Cell Types |
| 25 | Reflections on 24 Days of Learning |
| 26 | Mapping Immune Cells with Flow Cytometry |
| 27 | Fluorophore Fundamentals, panel Design and Spectral Flow Visualization |
| 28 | Flow Cytometry Controls: FMO, Isotype, and Experimental Validation |
| 29 | Visualizing Data: Dot Plots, Histograms and Gating Approaches |
| 30 | Troubleshooting Flow Cytometry |
| 31 | Cell Culture Essentials: Sterility and Handling |
| 32 | Preventing Contamination In Cell Culture |
| 33 | Understanding Adherent and Suspension Cell Culture |
| 34 | THP1 Differentiation into M1 and M2 Macrophages |
| 35 | Understanding ELISA: Mechanism and Uses |
| 36 | Mucus Secretion Assay: Beyond Classical ELISA |
| 37 | Western Blot Basics: From Gel To Band Interpretation |
| 38 | qPCR Basics: From RNA to Quantification |
| 39 | ELISpot Basics: Detecting Cytokine-secreting Cells with Precision |
| 40 | Fundamentals of Fluorescence Microscopy |
| 41 | Live Imaging: Watching Cells in Motion |
| 42 | Unlocking The Power of Multiplexing: From Ctokine profiling to CAR-T Potency |
| 43 | Cell Culture Crisis: Wh Are My Cells Dying |
| 44 | Lab Safety and Good Scientifc Practice |
| 45 | Lab Methods that Test Your Patience |
| 46 | Mouse Breeding Basics: Building Colonies, Tracing Generations |
| 47 | Drug Dosing for Mice: Calculations and Practical Considerations |
| 48 | Mouse Injections: IV, IP, SC and Beyond |
| 49 | Sampling and Visualization: Blood and Organ Concepts in Mice |
| 50 | Mastering Mouse Handling |
| 51 | Introduction to Cancer Immunology |
| 52 | Immune Checkpoints: Balancing Tolerance and Tumor Immunity |
| 53 | Adoptive Cell Therapy: Empowering The Immune Army |
| 54 | CAR-T Overview |
| 55 | FDA Approved CAR-Ts |
| 56 | CAR-T Side Effects |
| 57 | CAR-T in Solid Tumors: Overcoming The TME |
| 58 | Next Gen CAR-Ts: Synthetic Biology and Precision cell Therapies |
| 59 | Oncolytic Viruses: Turning Infection into Immunity |
| 60 | BiTEs: Bridging T Cells and Tumors for Precision Immunotherapy |
| 61 | Natural Killer Cell-based Therapies |
| 62 | Reprogramming The Defenders: Macrophage-based Cell Therapies |
| 63 | The Tumor Microenvironment: Layers of Immunosuppression and Resistance |
| 64 | Neoantigen Vaccines: Personalized Immunotherapy |
| 65 | Antigen Escape and Tumor Heterogeneity |
| 66 | Combination Regimens in Cancer Immunotherapy |
| 67 | From Bench To Bedside: Clinical Trial Stages in Modern Cancer |
| 68 | Investigational Medicinal Products in the EU |
| 69 | Individueller Heilversuch in Germany: When Innovation meets unmet Clinical Need |
| 70 | Recap of Immuno-oncology Series |
| 71 | Principles of Molecular Cloning |
| 72 | CRISPR in Immunology |
| 73 | Synthetic Biology beyond CAR-Ts: Mechanistic Principles |
| 74 | CAR Design Engineering |
| 75 | Optogenetics in Cellular and Immunotherapy |
| 76 | Organoids in Immunology: Modeling Human Immunity in the Dish |
| 77 | Microfluidics: Microenvironment Modeling and Precision Immunology |
| 78 | CyTOF: Mass Spectrometry for High-Dimensional Immune Profiling |
| 79 | Spatial Transcriptomics: Decoding Tissue-level Gene Expression Landscapes |
| 80 | Principles and Applications of Single-Cell RNA Sequencing in Immunology |
| 81 | Decoding Epigenetic Landscapes with ATAC-seq: from Regulatory Elements to Immune Function |
| 82 | Translating Methods to Immunology Research Questions |
| 83 | Hot vs. Cold Tumors: Immune-inflamed and Immune-excluded Cancers |
| 84 | Viruses as Vectors: Harnessing Viral Platforms for Gene Delivery in Immunology |
| 85 | Shuffling TCRs and Antibodies |
| 86 | Most Notable Immunotherapy Clinical Trials |
| 87 | Lab Automation: Streamlining Experiments for Modern immunology |
| 88 | 3D Printing in Biomedical Research |
| 89 | Essential Laboratory Acronyms in Biomedical Research |
| 90 | Immune-Cardiovascular Crosstalk in Health and Disease |
| 91 | Allergies: From Antigen Exposure to Immune Outcome |
| 92 | Mechanisms Matter: A Recap of Molecular and Cellular Immunology |
| 93 | Feeder Cells in Cell Culture: Supporting Cellular Growth, Maintenance, and Differentiation |
| 94 | Feeder-independent Cell Expansion: Advancing Scalable and Controlled Cell Culture Systems |
| 95 | T Cell Exhaustion vs. Senescence |
| 96 | The Reductionist Approach in Immunology: Dissecting Complex Systems into Mechanistic Units |
| 97 | Non-Hodgkin Lymphoma: Immunological Origins, Molecular Heterogeneity, and Therapeutic Implications |
| 98 | Immunology Abbreviations |
| 99 | Reflections after 99 Days of Teaching Immunology |
| 100 | What´s Next |